Bromothymol Blue and Bromocresol Purple as Indicators of pH Changes in Chromatophores of Rhodospirillum rubrum
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چکیده
منابع مشابه
Inhibition by nigericin of the light-induced pH change in Rhodospirillum rubrum chromatophores.
An important aspect of energy conservation in mitochondria, chloroplasts, and chromatophores is the temporal and sequential interrelationships between electron transport, proton movements (and the resultant proton gradient), highenergy intermediates or states, and the ultimate formation of adenosine 5'triphosphate (ATP).1-4 At present, two quite dissimilar alternative postulations are under con...
متن کاملPhotooxidase Activity of Heated Chromatophores of Rhodospirillum rubrum.
Van Niel's classical distinction between bacterial and green plant photosyntheses is based on the differences in the metabolism of the photooxidant (16). But direct measurement of the oxidizing power in bacterial photosynthesis is, at best, difficult with the usual spectrophotometric equipment. Light-induced oxidations of endogenous cytochromes have been demonstrated in whole cells and extracts...
متن کاملA disulfide photoreduction system in chromatophores of Rhodospirillum rubrum.
Chromatophores isolated from photosynthetic bacteria contain a disulfide-bonded repeating antigenic substructure that is serologically specific and present on the cell particles only during photosynthetic growth (l-3). This finding suggests that the structural antigen may also be a functional part of the photosynthetic electron transport system. This suggestion has been explored and supported b...
متن کاملThe Photo-oxidation of Succinate by Chromatophores of Rhodospirillum Rubrum.
1. The stoicheiometry of the photo-oxidation of succinate by chromatophores has been investigated with [2,3-(14)C(2)]succinate. It was found that there is a stoicheiometric relationship between the amount of succinate oxidized and the NAD reduced, and that fumarate is the only product of succinate oxidation. 2. The possibility of a direct hydrogen transfer from succinate to NAD in this reaction...
متن کاملBromocresol purple and ethyl red as indicators of events linked to changes in the energization of chloroplast membranes.
1. Two dyes, bromocresol purple and ethyl red, were tested for their ability to register solution and membrane changes resulting from the illumination of suspensions of isolated chloroplasts. 2. Bromocresol purple can measure the external (suspension) pH both rapidly and accurately if the following precautions are observed: (a) since bromocresol purple stimulates electron flow to oxygen, either...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1969
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1969.tb00678.x